Project Number: 1245-32000-098-15
Project Type: Reimbursable

Start Date: Oct 01, 2012
End Date: Sep 30, 2014

Objective:
ARS will expand its analysis of PRRS Host Genetics Consortium (PHGC) samples to address mechanisms of PRRS resistance/susceptibility and biologic responses to PRRS virus (PRRSV) infection. This grant will focus on PRRS viral persistence in tonsil and assess host immune and viral mechanisms involved in persistence. In addition it will update the PHGC database and website http://www.animalgenome.org/lunney/index.php for improved data resources for Consortia member usage and for public access and education.

Approach:
ARS has led the PRRS Host Genetics Consortium (PHGC) project the goal of which is to identify genetic determinants of resistance/susceptibility of commercial swine to porcine respiratory and the reproductive syndrome virus (PRRSV) infection and associated growth effects. ARS will apply for NPB funds to perform the proposed research on PRRS viral persistence. The objectives for this work are 1) Determine levels of PRRS viral RNA in porcine tonsils of PHGC pigs to assess PRRS persistence; 2) Assess tonsil tissue RNA for immune correlates of PRRSV persistence; and 3) Expand the capacity of the PHGC database and improve public/private interface. ARS will provide funds to the ISU COOPERATOR for their efforts for objective 3) to update the PHGC database and website.