Location: Vegetable Research
2013 Annual Report
2. Develop tomato translational genomic tools for virus resistance through RNA-Seq whole transcriptome analysis upon virus infection, functional analysis of the identified candidate genes for virus resistance, single nucleotide polymorphism (SNP) identification and association analysis, and molecular marker development to accelerate tomato breeding for resistance by pyramiding multiple virus resistance for tomato spotted wilt virus (TSWV) and tomato yellow leaf curl virus (TYLCV).
3. Assess the economical impact of viral diseases and benefits of using virus resistant cultivars to tomato growers including seed and chemical costs, market structure, formal and informal relationships between seed producers, tomato growers, packers and processors.
4. Develop an outreach program to communicate the outcomes of this project to stakeholders and the general public.
Using microRNA or a virus-induced gene silencing system to conduct functional gene analysis on the identified putative disease resistance candidate genes; Evaluate SNP mutations between resistant and susceptible parent lines and their association with disease resistance in the segregating populations (F1, F2, BC1); Molecular marker (CAPS) development; Selection and generation of breeding materials to achieve a multiple virus resistance through gene pyramiding using marker assisted selection; Evaluate potential economical losses due to virus disease infection; Conduct feasibility study on the need in developing a new technology and new cultivar; Determine economical benefits in adaptation of the new technology.
Create a project website for the understanding of a global distribution of viruses and viroids in tomato; Educate the public on the new technology for disease diagnosis, virus detection and disease resistance; Develop a workshop to teach tomato breeders in using marker assisted selection; Develop an extension booklet such as focus on tomato.
In benefit-cost analysis for prevention or reduction of viral diseases affecting tomato crops, two modeling components will contribute to this analysis. We will develop a bioeconomic model of tomato diseases. We will also develop a global trade model because the tomato market in the United States is heavily impacted by global market activities. Our model will be used to evaluate policy options to control tomato diseases. Developing this model requires substantial data. We are pursuing several sources by working closely with project scientists, seed companies and trade organizations to understand the plant pathology and specific concerns the industry has, which will be a key to accurate economic modeling. The project scientists visited several U.S. seed companies, which provided links to local industry groups, as well as, references to the American Seed Trade Association and the International Seed Federation. Working with these institutions will help us incorporate seed health, quality, and phytosanitary requirements into our model. Tomatoes are the second most important vegetable crop in the world after potatoes, and tomato seeds are traded globally, therefore our model must account for imports and exports around the world. Global Trade Information Services, Inc. (GTI) publishes monthly official government trade statistics for more than 80 countries. We are working to gain access to this database to incorporate this highly detailed trade information into our model to estimate key supply and demand parameters.
This is the first year progress report in the 4-year project. It is anticipated that once the project is completed, a comprehensive tomato virus and viroid inventory and a general global virus distribution map will be generated. The success of this project will lead us to determine reasonable phytosanitary requirements, to conduct proper risk assessment, and to recommend suitable disease management strategies to prevent or minimize viral infections affecting tomato productions.