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United States Department of Agriculture

Agricultural Research Service

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Research Project: DISTINGUISHING LIVE FROM DEAD ‘CANDIDATUS LIBERIBACTER ASIATICUS’ IN CITRUS TISSUE

Location: Citrus and Other Subtropical Products Research

Project Number: 6034-21000-016-02
Project Type: Trust

Start Date: Oct 01, 2012
End Date: Sep 30, 2014

Objective:
To distinguish live from dead cells of Candidatus Liberibacter asiaticus, presumed causal agent of citrus huanglongbing disease, in citrus tissue.

Approach:
The use of two DNA intercalating dyes (Ethidium Monoazide and Prodidium Monoazide) will be used to degrade DNA from dead cells while not affecting DNA contained in live cells, following treatment, DNA will be extracted and used in qPCR analysis; only DNA from live cells should amplify. Results with and without dye will be compared, parameters optimized and tested in citrus samples expressing various levels of HLB symptoms.

Last Modified: 11/27/2014
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