1a.Objectives (from AD-416):
The objective of the project is to further develop molecular diagnostic techniques we are currently working on to broaden their utility to the regulatory and diagnostic infrastructure in California and else where in the USA. Will also be collaborating with the CDFA diagnostic lab in Sacramento to ensure the techniques developed will be compatible with current workflow and to improve tech transfer to the end user of the technology.
1b.Approach (from AD-416):
We have developed a molecular diagnostic procedure for detection of Phytophthora in infected plant tissue where a genus specific marker is combined with a species-specific marker to identify species of regulatory concern (eg. P. ramorum). While markers have been developed for 12 species, this project will expand this to include other species that are important to agriculture. In addition to real-time PCR other technologies will be examined to see if they will be suitable for reducing the cost and time commitment for conducting the assays.
This agreement was established in support of Oobjective 2 of the parent project, the goal being to develop molecular diagnostic tools for the identificatioin of emerging diseases of vegetables and strawberry, and use these tools in the development of management strategies as alternatives to methyl bromide. TaqMan real time Polymerase Chain Reaction probes have been designed for the majority of the Phytophthora species under investigation and will be tested against a library of standardized isolates representing nearly all species in the genus. An isothermal amplification technique is under development which will simplify the ability to detect the pathogen.