1a.Objectives (from AD-416):
The objective of this project is to determine the seroprevalence of the zoonotic parasites Toxoplasma gondii and Trichinella spiralis in the national swine herd.
1b.Approach (from AD-416):
A statistically representative set of blood samples will be collected during the NAHMS 2012 National Swine Survey from randomly selected swine operations in the top hog producing states of the U.S. Samples will be assayed by ELISA for detection of antibodies to T. gondii and T. spiralis using commercially available test kits. Putative positive samples will be retested to confirm results. Results will be tabulated to determine a national prevalence of T. gondii and T. spiralis in the U.S. swine herd.
The ARS laboratory is conducting a serological survey of Toxoplasma and Trichinella infection using sera and management data collected during the 2012 National Animal Health Monitoring Swine Survey (NAHMS). A statistically representative set of blood samples has been collected during the 2012 NAHMS Survey from randomly selected swine operations in the 17 top hog producing states in the U.S. Samples are currently being assayed by ELISA for detection of antibodies to T. gondii and T. spiralis. Four thousand of the 7300 samples have been tested for both pathogens. Final results will be tabulated after completion of testing to determine a national seroprevalence of T. gondii and T. spiralis in the U.S. swine herd.