Location: Nematology Laboratory
Project Number: 1245-22000-284-00
Start Date: Dec 14, 2012
End Date: Dec 13, 2017
1. Nematodes that infect alfalfa and other forage legumes, grasses, rotation crops, or potential weed hosts will be identified using molecular markers including ribosomal, mitochondrial, Hsp90, and other nuclear genes. 2. New diagnostic assays, including RFLPs, and conventional or real-time PCR assays will be developed for Meloidogyne spp., Pratylenchus spp., Ditylenchus spp., or others affecting these plants. 3. Molecular information from the diagnostic work will be integrated with morphological data and information regarding biogeography, pathogenicity, and host range to generate new and improved phylogenetic schemes. 4. Molecular diagnostic tools to aid in screening resistant alfalfa germplasm will be developed to detect and quantify nematodes from infected alfalfa tissue. 5. Fungal-feeding nematodes and their associated fungal endophytes will be isolated from alfalfa, forage grasses, or weeds and identified and characterized by morphological and molecular methods. 6. Fungal-feeding nematodes will be cultured with fungal symbionts in lab cultures to determine suitability of endophytes to support nematode growth and reproduction.