2013 Annual Report
1a.Objectives (from AD-416):
1)develop breeding populations for use in genetic improvement of Brassica napus, and for use in validation of molecular markers arising from collaborative genomic studies;.
2)grow out in the field the genetic diversity panel a world collection of Brassica napus for use in genome wide association studies; and.
3)grow out in the Idaho location the multi-species trials (stress trials) as part of the broader US stress trials study, and provide phenotypic data needed for ALMANAC modeling toward redesign of oilseed to biofuels conversion.
1b.Approach (from AD-416):
New and improved germplasm and breeding populations from ongoing ARS and University of Idaho breeding programs will be established in small and large scale research plots. Small plots will be scored for a variety of phenotypic traits, including yield, height, leaf area, canopy temperature, etc. Seed samples will be collected from these plots and sent to Peoria, IL for detailed oil analysis. Large scale plots will be planted to determine relative commercial performance of multiple oilseed species and breeding lines.
Brassica napus (L.) is one of the most productive oilseed species, existing as either a winter or spring annual crop, with diverse oil compositions. To increase the economic return on investment in use of B. napus as a source of biofuel, genetic improvement for oil yield and quality across diverse environments is needed. In that light, we are creating a collection of 800 genetically diverse accessions (diversity panel) from the widest available germplasm worldwide to use as a resource for genetic improvement.
This research relates to objective 1 of the inhouse project, “Develop genotyping-by-sequencing methods for diverse cotton, oilseed, and industrial crop germplasm, and map genetic markers for economically and agronomically important traits in these crops". This project is associated with oilseed production, and builds upon our parent project by performing genome sequencing of, and molecular marker identification within, a large Brassica napus diversity panel.
Our collaborator in Idaho has created and grown out the first year’s genetic diversity panels, and collected leaf samples to send to KeyGene, Inc. for Deoxyribonucleic acid (DNA) sequencing. Our collaborator has also created Recombinant Inbred Lines (RILs) that will be used later to test and validate marker identity from Single nucleotide polymorphisms (SNPs) arising from the genomtyping by sequencing efforts. The RILs are being grown to advanced inbred generations. The collaborator is also one of 10 sites in the US growing out the species trials (called stress trials) that will soon be used for ALMANAC modeling.