1a.Objectives (from AD-416):
To characterize the metabolomic profile between resistant and susceptible bean lines to discover metabolomic profiles associated with host plant resistance, and characterize resistant and susceptible lines for metabolites known to be associated with the host pathogen interaction in previous studies. Discovery of metabolites associated with resistance could be used to validate the association in existing RIL populations developed from parental lines in this study.
1b.Approach (from AD-416):
Ten parental lines from five RIL populations that segregate for resistance to Sclerotinia were selected for metabolite analysis. Leaves from all 10 accessions will be simultaneously inoculated in a detached leaf assay, after which metabolites will be extracted at three timepoints. Metabolites will be detected and quantified using ultra performance liquid chromatography (UPLC-MS), GC-MS, and UPLC-MS/MS. This study consists of both a non-targeted (UPLC-MS, GC-MS) and targeted (LC-MS/MS) determination of metabolite content. This allows for both the discovery of novel metabolic processes, as well as a confirmation of metabolites previously shown to be involved in disease resistance. Metabolite variation will be assessed by quantitative differences between resistant and susceptible varieties at 0 (basal metabolite differences), 24, and 72 hours post-infection (induced metabolite differences). Metabolite variation will additionally be analyzed in the context of biochemical pathways to determine the relationship between resistance/susceptibility and host metabolism.
Results will be shared with colleagues via progress reports, refereed publications, and meetings. Implications from this research should ilucidate metablolites that are associated with resistance and quantify the level of association.
This project was initiated on September 1, 2012 and the long-term objective is to validate the effects of a resistant QTL on linkage group B7 in the common bean, and to combine forms of genetic resistance using molecular markers linked to resistance genes found in Andean common bean line G 122 with resistance genes from the related species P. coccineus.