2013 Annual Report
This is a final report for this project. Funds from this project have been used to create a Specific Cooperative Agreement (SCA) with University of Hawaii, Manoa. In this collaborative effort, tools to identify the source of intercepted medfly or oriental fruit fly are being developed as well as tools for distinguishing Vienna lines of medfly from wild sources (Vienna specific markers). In addition, the invasion history of prior interceptions of medfly and oriental fruit fly is being analyzed. To date, a protocol for multigene sequencing using a combination of dynamic arrays and high throughput sequencing has been developed for both medfly and Oriental fruit fly. This data is being used to evaluate the invasion history of these flies. In addition, in order to identify marker in Vienna lab lines, a crossing experiment has been performed between Vienna 7 lab line flies from the California Department of Food and Agriculture (CDFA) rearing facility and an inbred lab line of medfly maintained at USDA, Pacific Basin Agricultural Research Center (PBARC), Hilo, Hawaii. In this F1 backcross design, we have generated an F4 population that exhibits the white pupae and temperature sensitive lethal traits present in female Vienna 7 flies, despite being backcrossed to the inbred lab line. This line is being used now for construction of a linkage map utilizing reduced representation sequencing methods.