1a.Objectives (from AD-416):
Five breeding clones from the USDA-ARS potato breeding program have been identified as having sufficient merit for the production of tissue culture nuclear plantlets free of pathogens. Tissue culture plantlets will then be used for the production of certified seed to allow their evaluations in the National Fry and Chip Processing Trials as part of the SCRI Acylamide Reduction Grant.
1b.Approach (from AD-416):
Aberdeen potato breeding clones A00206-1C, A02138-2, A02424-83LB, A02507-2LB, and A03921-2 will have viral and bacterial pathogens removed using plant tissue culture techniques, allowing the production of certified seed for the clones’ evaluation in the National Fry and Chip Processing Trials—components of the SCRI Acrylamide Reduction Grant.
Tuber of five Aberdeen potato breeding clones, A00206-1C, A02138-2, A02424-83LB, A02507-2LB, and A03921-2 were provided to the University of Wisconsin for their establishment in tissue culture and for the subsequent elimination of virus and bacterial pathogens, allowing for the production of certified seed for commercial evaluations. Clean plantlets of all five breeding clones were obtained and two clones, A02507-2LB and A02138-2, were subsequently identified in 2013 as promising, low-acrylamide breeding clones suitable for commercial scale evaluations as a component of the SCRI Acrylamide Reduction Grant. Plantlets were sent for the greenhouse production of approximately 20,000 mini-tubers of each breeding clone in 2013 to allow for the generation of certified seed in the field in 2014. The research conducted contributes to Objective 1 of the ARS parent project.