1a.Objectives (from AD-416):
To characterize the duration of infection of various serovars and variants of Salmonella within peripheral lymph nodes of cattle following intradermal inoculation.
1b.Approach (from AD-416):
Following successful models developed within the Salmonella Working Group, we will enroll 36 animals and transdermally inoculate various serovars and variants (i.e., clade A versus clade B). At nine time points, calves will be harvested and Salmonella presence evaluated both qualitatively and quantitatively. Prior to strain selection, consultation will occur within the Salmonella Working Group. Various models (such as survival analysis and stochastic simulation) will be used to estimate duration of infection.
This is a new project, with the goal of determining the duration of Salmonella infection in the peripheral lymph nodes of cattle following transdermal inoculation. In FY 2013, project work established that lymph nodes with quantifiable populations of Salmonella were produced soon after a single inoculation time point, and nodes were detected with the Salmonella strain throughout the experimental period. Work by this project has developed important new information on the duration of infection of Salmonella within the peripheral lymph nodes that will facilitate the design and implementation of successful pre-harvest mitigation strategies. This project expired in FY 2013, but parent project scientists will continue work with the cooperator in order to more definitively establish the dynamics and duration of Salmonella lymph node infection in cattle.