Project Number: 1235-52000-060-18
Start Date: Sep 16, 2012
End Date: Sep 30, 2015
Authentic samples of Black Cohosh (Actaea racemosa), Echinacea (Echinacea angustofolia and Echinacea purpurea), and Echinacea hybrids will be collected. Specific gene regions will be sequenced (“DNA barcodes”) and/or microsatellites will be genotyped (“DNA fingerprints”). Additionally, chemical fingerprints will be obtained based on chromatographic profiles from ultra high performance liquid chromatography (UHPLC) and spectral fingerprints from direct injection high resolution mass spectrometry (HRMS). Metabolomic profiles will be obtained by combining the chromatography and mass spectrometry for UHPLC-HRMS. Chemometric pattern recognition methods will be applied to the fingerprints to discriminate between species and hybrids. Chemical fingerprints will be correlated with the DNA barcodes and/or fingerprints. The results of these studies will be incorporated into a publicly available database.