1a.Objectives (from AD-416):
The objective of this cooperative research project is:. 1)to develop DNA based hypotheses of relationships (phylogeny) for fruit flies and other flies from Peru; and. 2)to investigate the use of DNA sequence data to diagnose these species.
1b.Approach (from AD-416):
ARS and the Centro de Ecologia y Biodiversidad will collect samples of Peruvian flies and jointly train students to make additional collections. ARS will extract, sequence, and analyze DNA from these samples for use in ongoing systematic studies of fruit flies and other flies, including analyses of phylogenetic relationships and diagnosis of species. Results and data will be shared with CEBIO and made available to the scientific community.
Two trips were made to Peru to obtain samples of fruit flies. On the first trip (Nov 2012) traps were installed at a field station and arrangements were made for a student to collect the samples. On the second trip (Jan-Feb 2013), the samples were obtained, and additional samples were collected from a second area in a different valley. Approximately 35 species of fruit flies were collected. DNA will be extracted from these samples and analyzed to find new characters to distinguish these fruit fly species. We met with our Collaborators at the Universidad Nacional San Antonio Abad del Cusco and Amazon Conservation Association to discuss ongoing and future collaboration.