Project Number: 5012-22000-021-12
Start Date: Sep 01, 2012
End Date: Mar 31, 2014
Soybean samples will be collected from field sites with various treatments including pesticides (seed and foliar applied fungicides and insecticides, and seed nematicides), plant defense elicitors, and certain cover crops prior to soybean. These samples will be assayed for various pathogens and pests of soybeans. Some of these assays will be completed through traditional approaches of isolation and enumeration; others will be assayed using quantitative detection of target DNA using PCR. Most of the pathogen and pest assay data will be related to field observations of diseases and pests, and to the yield of soybeans in the experimental plots. Objective 2: Seeds of soybean germplasm including commercial cultivars and accessions (both soybean and wild perennial accessions from the USDA Soybean Germplasm Collection) will be assembled for resistance evaluation to various soybean pathogens and pests. These evaluations will be done using common greenhouse inoculation techniques, as well as other techniques like detached leaves or leaf disks that are not yet standard prodecures. The most resistance genotypes will be retested in the field (if possible) and again using different strains of the pathogen and/or pest to confirm resistance.