1a.Objectives (from AD-416):
To obtain transcriptomic date of various Phakopsora pachyrhizi isolates during infection and from germinating urediniospores.
1b.Approach (from AD-416):
Advances in nucleic acid sequencing technologies (i.e., "next generation" sequencing) provide a fast and cost-effective way to analyze gene expression. In this study, plants infected with Phakopsora pachyrhizi (Asian soybean rust) and ungerminated P. pachyrhizi urediniospores will be used as the source of the RNA. Next generation sequencing protocols will be used to identify the RNA transcripts present in the samples. The resulting sequence data will be provided to USDA-ARS for the study of genes important for fungal virulence and the triggering of plant defense responses.
Advances in nucleic acid sequencing technologies (i.e. "next generation" sequencing) provide a fast and cost-effective way to analyze gene expression. This technology is being used to analyze gene expression in soybean leaves infected with Phakopsora pachyrhizi (Asian soybean rust). Heavily infected soybean leaves were provided to collaborating scientists who extracted high-quality RNA for sequencing. Multiple isolates of the pathogen were used. The collaborator is performing the vector construction and sequencing. Sequencing and bioinformatic subtraction of host gene sequences from the data is in progress. The final sequence dataset will be provided to USDA-ARS upon completion, where it will be analyzed for genes important for fungal virulence and the trigging of plant defense responses.