SCREENING AND CLONING OF RESISTANCE RELATED GENES BY RNA-SEQ IN HUANGLONGBING (HLB) RESISTANT AND SUSCEPTIBLE CITRUS BREEDING LINES
Subtropical Plant Pathology Research
Project Number: 6034-22000-039-26
Start Date: May 01, 2012
End Date: Apr 30, 2015
1. Generate transcriptome profiles of both susceptible and resistant citrus responding to huanglongbing (HLB) infection.
2. Identify key resistant genes from differentially expressed genes and gene clusters between the HLB-susceptible and HLB-resistant plants.
3. Create transgenic citrus cultivars with new constructs containing the resistant gene(s).
Using RNA-Seq with next generation of sequencing technology, and intensive bioinformatics to identify key resistant genes from susceptible and resistant citrus plants in response to Candidatus Liberibacter asiaticus infections, and using routine transformation techniques to create transgenic citrus plant with the select resistant gene.