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United States Department of Agriculture

Agricultural Research Service

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Research Project: Genomics Based Approach for Identifying Genetic Traits and Markers Linked to Disease Resistance in Brassica Spp.

Location: Sunflower Research

Project Number: 5442-21220-026-06
Project Type: Specific Cooperative Agreement

Start Date: Sep 15, 2012
End Date: Sep 14, 2017

Objective:
1) Identify molecular markers linked to Sclerotinia and blackleg disease resistance in Brassica spp. and 2) screen for genetic traits leading to enhanced disease resistance in canola.

Approach:
Next generation sequence technologies will be used to screen breeding populations of Brassica spp. for molecular markers associated with disease resistance including (1) sequencing double haploid (DH) PI lines (parental lines resistant or susceptible to Sclerotinia), several lines from an F6 breeding population with resistance to Sclerotinia, and Westar (a susceptible variety); (2) conduct Genome Based Sequencing (GBS) on an F2 population developed from DH parents and a bi-parental mapping F2 population to identify single nucleotide polymorphisms (SNPs). SNPs and other molecular markers resulting from this sequencing project will be used to genotype breeding populations that have been phenotyped based on exposure to fungal pathogens causing Sclerotinia and blackleg disease.

Last Modified: 9/10/2014
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