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United States Department of Agriculture

Agricultural Research Service

Research Project: Evaluation of Carbohydrate Sources in Yeast-Fermentation Co2 Generators on Mosquito Surveillance

Location: Mosquito and Fly Research Unit

Project Number: 6036-32000-050-10
Project Type: Trust

Start Date: Jan 01, 2014
End Date: Dec 31, 2015

Objective:
1. Build a device that generates carbon dioxide (CO2) through yeast-fermentation and compare the synergistic attractiveness of yeast metabolites and volatiles present in honey, molasses, and sugar to wild and laboratory mosquitoes through overnight trapping. 2. Analyze the distinct volatiles off-gassing from the different carbohydrate sources as they are being fermented by yeast, and compare them against volatiles assessed in prior mosquito olfactory response research. 3. Develop and publish a concise method that can be followed by entomologists and public health specialists to deploy the yeast-fermenting CO2 generator (YFG) in remote areas.

Approach:
The yeast-fermenting CO2 generator (YFG) will be used to ferment an array of carbohydrate sources. The gasses released will be used to attract wild and laboratory populations of mosquitoes to baited CDC traps. Trapping experiments will use three sets of a Latin square design with four treatments (sucrose, honey, molasses, and dry ice) rotated through four locations over four nights of consecutive trapping. Mosquitoes collected from the wild will be identified to species and counted. Non-target organisms will also be counted, but identified to family. Known numbers of laboratory reared mosquitoes will be released in large outdoor screened cages at CMAVE. One YFG baited CDC trap will be placed at each of four corners and rotated over four consecutive nights of trapping. Trap nights will begin at 1700 and end the following morning at 0800. Gasses released by fermentation will be collected and measured to determine volume in relation to carbohydrate concentration for each treatment. In addition, the gasses will be analyzed to determine their components and concentrations by GCMS.

Last Modified: 12/21/2014
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