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United States Department of Agriculture

Agricultural Research Service

Related Topics

Research Project: Determination of Attractive Host Plant Volatiles and Sex Pheromones of Asian Citrus Psyllid Using Eags and Gc-Ead

Location: Subtropical Insects and Horticulture Research

2013 Annual Report

1a.Objectives (from AD-416):
Develop an attractant for Asian citrus psyllid.

1b.Approach (from AD-416):
Gas chromatograph-coupled electroantennogram analysis of host plant volatiles and putative pheromone compounds. Laboratory behavioral assays.

3.Progress Report:

This project is directly related to Objective 1, Develop semiochemical-based control methods for citrus pests, particularly Asian citrus psyllid (ACP).

The first successful gas chromatograph-coupled electroantennograms (GC-EAD) were produced using Asian citrus Psyllid (ACP) antennae. GC-EAD was used in conjunction with GC-Mass Spectrometry (GC-MS) to identify volatile compounds that elicited antennal responses. Recording from ACP antennae has been technically challenging and it is still not clear that ACP adults rely upon chemical cues to locate host plants or conspecifics. We have now identified four compounds that elicit large depolarization or hyperpolarization of antennal chemoreceptors. Two of these compounds appear to be the products of oxidative degradation of a common plant volatile. These compounds and their blends will be evaluated in behavioral assays in an attempt to identify attractants and/or repellents. Such compounds would be useful for development of new control methods for citrus growers.

Last Modified: 1/29/2015
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