Project Number: 1245-21000-190-27
Project Type: Specific Cooperative Agreement

Start Date: Jun 26, 2012
End Date: Aug 30, 2014

Objective:
To identify and characterize late blight resistance mechanisms from two diverse parents by RNA-Seq technology and combine multiple resistance mechanisms into a single elite donor line. Reduce the impact of late blight through the use of resistant varieties and reduced fungicide programs; Conduct late blight resistance phenotyping of advanced breeding lines and a breeding population for future use in genetic marker development.

Approach:
Intercross late blight resistant clones of Solanum phureja-S. stenotomum with late blight resistance backcross introgression line of S. tuberosum-S. bulbocastanum. Construct RNA-Seq library for resistant and susceptible parents. Sequence transcriptome libraries.