SIMULTANEOUS IMPROVEMENT OF FIBER QUALITY AND HEAT TOLERANCE
Plant Stress and Germplasm Development Research
2012 Annual Report
1a.Objectives (from AD-416):
The research proposed for 2012 will focus on cotton reproductive stress tolerance and the further development and characterization of recombinant inbred populations of PHY72(F) X STV474(M) and STV474(F) X PHY72(M). The proposed research will focus on understanding the genetic control of cotton pollen dehydration tolerance and the underlying biochemical reasons for observed differences in stress tolerance.
1b.Approach (from AD-416):
The PHY72(F) X STV474(M) and STV474(F) X PHY72(M)recombinant populations will be advanced to the F7 generation. Flower shape and pollen dehydration tolerance will be re-evaluated on F7 plants. DNA will be harvested and sent to the USDA-ARS Cotton Fiber Bioscience Research Unit, New Orleans, LA, for mapping studies. Lint samples will be harvested and sent to CI for quality evaluation. If we are able to obtain sufficient seed, we will plant the population in the field for initial yield evaluations.
The PHY72(F) X STV474(M) and STV474(F) X PHY72(M) recombinant populations have been advanced to the F7 generation. Both populations are in the greenhouse for seed increases. Flower shape has been re-evaluated on the F7 plants. DNA has been harvested for mapping studies. Lint samples have been harvested for quality evaluation. Because we were able to obtain sufficient seed, we planted the STV474(F) X PHY72(M) population in the field for initial water stress responses and yield evaluations. A commercial check (FM9180) was used in the augmented design for the field. Final samples have been collected and shipped to a cooperator for characterizing the lipids of the pollen from dehydration-tolerant and -susceptible pollen.