Location: Food and Feed Safety Research
Project Number: 3091-32000-030-64
Start Date: Nov 01, 2012
End Date: Dec 31, 2015
A multi-pronged inoculator (e.g., 10-lancet) allergy skin-testing device is inoculated with a Salmonella broth and applied with mild pressure to specific regions of the bovine's hide that are drained to region-specific lymph nodes. Different subtypes or serotypes of Salmonella can be applied to different regions of the animal. This process may be repeated at the time of initial inoculation or at subsequent days. At various time points after inoculation, the calves are sacrificed and the lymph nodes recovered and Salmonella presence can be determined or quantified using various methods. Various statistical approaches (such as survival analysis) can be performed to estimate duration of infection. Moreover, candidate interventions can be applied to a portion of the calves to evaluate any impact on the duration of infection within peripheral lymph nodes.