Location: Grain, Forage & Bioenergy Research
Project Number: 5440-21000-031-07
Start Date: May 01, 2012
End Date: Apr 30, 2017
WSMV, TriMV, and HPV are economically important viruses in the Great Plains region of the USA, and all three viruses are vectored by wheat curl mites. Wheat cultivars with a broad-spectrum resistance to multiple viruses are ideally suitable for the effective management of viral diseases in wheat. It has been demonstrated that a transgene with separate short hairpins (200-300 nucleotides) derived from different viruses can provide resistance to multiple viruses. Hence, we proposed to develop hairpin constructs from the conserved regions of WSMV, TriMV and HPV genomic RNA sequences between the maize ubiquitin promoter and NOS-terminator sequences in a binary vector. Similarly, putative microRNA precursor sequences from the genomic RNAs of WSMV, TriMV and HPV will be identified and cloned in a binary vector between the maize ubiquitin promoter and NOS-terminator. The binary plasmids containing expression cassettes comprising the hairpin or microRNA precursor sequences from WSMV, TriMV and HPV will be used to transform wheat. The transformants containing hairpins or microRNA precursor sequences will be screened for resistance against WSMV, TriMV and HPV.