2012 Annual Report 1a.Objectives (from AD-416): Perform genetics analysis of high oleic acid lines. 1b.Approach (from AD-416): We plan to cross PI 603452 (GmFAD2-1a mutant) with each of PI 210179, PI 283327, PI 567189A, PI 567189B, and PI 578451 (GmFAD2-1b mutants) identified by our group to create a double mutant line containing even higher levels of 18:1. Simultaneously, single nucleotide polymorphism markers will be designed that correspond to each GmFAD2-1 mutation to increase the precision and speed by which the resultant high oleic phenotype can be introgressed into elite germplasm. For each cross, segregating populations will be developed and field trials studies will be conducted to quantify the extent by which the combinations of these new mutations enhance 18:1 content, and also establish the stability of the trait across different environments. The two mutant alleles will also be backcrossed to a high yielding line. 3.Progress Report: This project is related to Objective 2 of the in-house project: To discover novel genes/alleles in soybean for seed composition, determine their inheritance, determine genomic location, transfer to adapted germplasm, and release. This new research project is a continuation of the research project 6645-21220-012-37S and has been active only since April 1, 2012. Populations derived from the crosses between PI 603452 (FAD2-1A) and PI 210179, PI 283327, PI 567189A, PI 567189B, and PI 578451 (GmFAD2-1B mutants) were planted in the field and tissue has been collected to extract DNA and genotype the populations with Single Nucleotide Polymorphisms (SNP) markers that target the two high oleic loci.