1a.Objectives (from AD-416):
Perform genetics analysis of high oleic acid lines.
1b.Approach (from AD-416):
We plan to cross PI 603452 (GmFAD2-1a mutant) with each of PI 210179, PI 283327, PI 567189A, PI 567189B, and PI 578451 (GmFAD2-1b mutants) identified by our group to create a double mutant line containing even higher levels of 18:1. Simultaneously, single nucleotide polymorphism markers will be designed that correspond to each GmFAD2-1 mutation to increase the precision and speed by which the resultant high oleic phenotype can be introgressed into elite germplasm. For each cross, segregating populations will be developed and field trials studies will be conducted to quantify the extent by which the combinations of these new mutations enhance 18:1 content, and also establish the stability of the trait across different environments. The two mutant alleles will also be backcrossed to a high yielding line.
This project is related to Objective 2 of the in-house project: To discover novel genes/alleles in soybean for seed composition, determine their inheritance, determine genomic location, transfer to adapted germplasm, and release. We generated BC3F1 seeds from 6 crosses of recurrent parents NC-Tinius and NC-Burton to lines carrying a FAD2-1A mutation from PI603452 and FAD2-1B mutations from 6 PIs in the greenhouse. However, due to low temperature and short days, some male and female plants became cleistogamous and we are not certain that we will have enough BC3F1 seeds from all crosses. We harvested and threshed F3:4 seed from 14 interconnected high oleic acid quantitative trait loci (QTL) mapping populations that were homozygous or heterozygous for the FAD2-1A mutation. We checked that the parents and the progeny of each populations corresponded to each other. We requested seed from each parent from germplasm resources information network (GRIN) to check that the parents of our populations are the same as the GRIN system to correct mistakes and discard populations that do not match to GRIN.