2012 Annual Report
Over the next 5 years our research will focus on the following objectives:
Objective 1: Determine the environmental fate of non-toxigenic strains of Aspergillus (A.) flavus using molecular tools. Sub-Objective 1a. Compare DNA sequence information from current biocontrol strains with indigenous strains to identify unique molecular markers. Sub-Objective 1b. Monitor the post-release spatial and temporal distribution of A. flavus biocontrol strains in corn fields.
Objective 2: Determine the mechanisms of biocontrol efficacy with non-toxigenic Aspergillus flavus strains.
Objective 3: Optimize a water dispersible formulation and application procedure for the use of non-toxigenic strains of Aspergillus flavus for biocontrol of mycotoxins in corn. Sub-Objective 3a. Develop and evaluate water dispersible granular (WDG) formulation of non-toxigenic Aspergillus flavus strains. Sub-Objective 3b. Evaluate the Accu-Flo™ spray nozzle for aerial application of biological materials. Sub-Objective 3c. Determine the effect of timing of the application of K49 and Afla-Guard® as a WDG formulation. Sub-Objective 3d. Compare the effect of a combined inoculum of K49 and Afla-Guard® to these inoculants used separately.
Objective 1: HiSEQ Illumina-based sequencing was performed on 2 commercially available and 1 experimental biocontrol strain and is being compared to a native toxin-producing strain. Additional sequencing has been performed on 150 Mississippi-native strains to evaluate indigenous diversity. The first season of the four-year study has been planted on a private farm and biocontrol strains have been applied. Soil samples were collected and A. flavus strains were isolated as a reference for the Aspergillus (A.) flavus population prior to introduction of biocontrol strains.
Objective 2: An assay was developed for the in vitro study of competition by measuring fungal growth and aflatoxin production.
Objective 3: Fungal spores of both strains K49 and Afla-Guard were mass produced as a Water Dispersible Granule (WDG) formulation in 2012. Research on biological methods for controlling aflatoxin in corn is progressing with minimal difficulties such as weather effects, lands, and lack of equipment availability in 2012. Field studies in several locations for optimizing WDG formulation for improvement of delivery of both non-toxigenic Aspergillus flavus K49 and Alfa-Guard strains is in its first season. These studies included rate of application; time of application; and mixed vs sole application of both strains of WDG formulation. The WDG formulation of both strains was provided to collaborator to perform preliminary evaluation of droplet characteristics in 2012 in order to determine useful data that could be used for adjusting droplet size in field aerial application of WDG by plane.
Some of the research on this project is being done in cooperation with scientists at other institutions. Research in objective 3 is being conducted by Syngenta under a specific cooperative agreement and a CRADA between ARS and Syngenta. Separate reports summarize work completed under these agreements (Subordinates 6402-42000-005-02T and 6402-42000-005-04T, respectively). Research in objective 3 is being conducted by Mississippi State University scientists and ARS scientists under a Specific Cooperative Agreement with Mississippi Corn Promotion Board (MCPB). A separate report summarizes work completed under this agreement (Subordinates 6402-42000-005-05T and 6402-42000-005-03T).
Accinelli, C., and Abbas, H.K. 2011. New Perspectives for the Application of Bioplastic Materials in the Biocontrol of Aspergillus flavus in Corn. Toxin Reviews. 30(2-3):71-78.
Trucksess, M.W., Abbas, H.K., Weaver, C.M., and Shier, W.T. 2011. Distribution of aflatoxins in shelling and milling fractions of naturally contaminated rice. Journal of Food Additives & Contaminants. 28:1076-1082.
Manning, B.B., Abbas, H.K., Wise, D.J., Peterson, B.C. 2011. Channel catfish, Ictalurus punctatus, fed diets containing aflatoxin from moldy corn do not experience increased mortality after challenge with Edwardsiella ictaluri. Journal of the World Aquaculture Society. 42(4):598-602.
Jiang, T., Zhou, B., Luo, M., Abbas, H.K., Kemerait, R., Lee, R.D., Scully, B.T., Guo, B. 2011. Expression analysis of stress-related genes in kernels of different maize (Zea mays L.) inbred lines with different resistance to aflatoxin contamination. Toxins. 3(6):538-550.
Bruns, H.A., Ebelhar, M.W., Abbas, H.K. 2012. Comparing single-row and twin-row corn production in the mid south. Crop Management. 1:1-48, doi:10.1094/CM-20120404-01-RS.
Weaver, M.A., Zablotowicz, R.M., Krutz, L.J., Locke, M.A., Bryson, C.T. 2012. Microbial and vegetative changes associated with development of a constructed wetland. Ecological Indicators. 13:37-45.
Chang, P.-K., Abbas, H.K., Weaver, M.A., Ehrlich, K., Scharfenstein, L.L., Cotty, P.J. 2012. Identification of genetic defects in the atoxigenic biocontrol strain Aspergillus flavus K49 reveals the presence of a competitive recombinant group in field populations. International Journal of Food Microbiology. 154:192-196.