2013 Annual Report
During the period covered by this report, project personnel completed additional caged vector experiments using western flower thrips, including transmission tests with Tomato chlorotic spot virus (TCSV), which is closely related to GRSV. Additional experiments are underway to determine which host plants are most important for thrips acquisition (and subsequent transmission) of GRSV and TCSV. Results of parallel thrips transmission experiments with GRSV and TCSV have extended previous project work and continue to represent substantial progress in project efforts to determine which local thrips species are important for GRSV and TCSV transmission in Florida.
Field and transplant house sampling of tomato, and also pepper, eggplant, potato and solanaceous weeds has continued with over 164 samples collected and tested during this reporting period. GRSV, TCSV and TSWV have all been detected in many locations in south Florida. Collectively, these results suggest that like GRSV, TCSV has become established in south Florida and make it important to continue to monitor tomato production for this newly detected relative of GRSV. An experimental host range determination for TCSV has continued in parallel to project work on GRSV. Collectively these results represent substantial progress in project efforts since TCSV is one of the parents of the Florida GRSV isolate. Weekly thrips counts and GRSV/TCSV incidence data from disease hotspots have been collected. These data will be used by other project personnel to prepare distribution maps of thrips and GRSV/TCSV for subsequent analysis. This activity will continue each season through the conclusion of the project. Field transect studies have continued in GRSV/TCSV hotspots.
Resistant and susceptible tomato cultivars for field tests have been identified from greenhouse host range testing for use in field trials in the fall growing season.