2012 Annual Report 1a.Objectives (from AD-416): To decrease the amount of acrylamide in processed potato products. 1b.Approach (from AD-416): We will focus on indentifying low asparagine or high antioxidant potatoes and determining to what extent this reduces acrylamide formation. 3.Progress Report: This is the final report for this project. We are optimizing a gas chromatography-mass spectometry (GCMS) technique that will allow us to quantitate asparagine and sugar levels in diverse potato germplasm. Two criteria that we are trying to achieve are maximal throughput and reasonable cost, both of which relate to sub-objective 1.A. of the in-house project, "Identify germplasm with a range of expression of phytonutrients, study inheritance, identify associated markers, and produce superior parents". This method is also capable of measuring over a hundred additional compounds and this could be useful for understanding how other metabolites influence asparagine or sugar metabolism. We’ve been working on generating potatoes with different amounts of antioxidants so that we can evaluate to what effect tuber antioxidant capacity can mitigate acrylamide formation.