1a.Objectives (from AD-416):
Conduct experiments to improve the oil profile of soybean seeds. Evaluate new combinations of fatty acid profiles with the high oleic acid trait. This project will produce soybean germplasm in a high oleic acid background with additional oil profile traits to determine the effect of different combinations of genes to produce desirable fatty acid profiles in the oil. Targets include high oleic acid (HO) plus low palmitic acid, HO plus low palmitic acid and low linolenic acid, HO plus high stearic acid. The genes controlling the traits will be merged using the latest molecular marker technology, and germplasm will be produced and tested in an appropriate field environment for the fatty acid profiles of the seeds.
1b.Approach (from AD-416):
Molecular genetics and plant breeding experiments will be conducted to combine desired mutant alleles controlling the accumulation of different fatty acids in the seed oil. Different crosses will be made between parental lines containing the two genes for the high oleic acid trait and parental lines containing other fatty acid profile traits, including low palmitic acid and elevated stearic acid. Genotyping selections with existing or newly developed molecular marker assays will be performed on the F1 and F2 progeny. Standard population development will be used to advance selected lines to obtain materials for phenotypic evaluation. Field tests will be set up to compare different combinations of alleles important for the seed oil fatty acid profiles to evaluate the effect of each of the alleles working in combination. Laboratory, field, and winter nursery are all components of this research.
The objective of this project is to determine the molecular genetic basis for increased high quality seed protein content in soybean seeds. The objective links to the in-house project (to develop the molecular basis for modification of the fatty acid components of soybean oil and anti-nutritional components in soybean meal to use in accelerated breeding programs) by exploring the relationship between protein content and quality and by exploring the molecular genetic basis for protein quality traits. We completed one aspect of the project, determining the protein content (via total nitrogen measurement) of a set of recombinant inbred lines (RIL) segregating for the high protein trait. We began development of an RIL population to examine the effect of different alleles of the high protein donor quantitative trait loci. We developed populations with selection for seed composition traits that will be segregating for the high protein trait. We are evaluating the effect of nitrogen feeding on the protein content and quality of selected genetic high protein lines. We identified a soybean line with a novel seed composition phenotype related to the accumulation of phytic acid, and we are characterizing lines segregating for the trait.