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United States Department of Agriculture

Agricultural Research Service

Research Project: A Systems Approach to Control Gastrointestinal Nematodes in Organic Small Ruminant Production: Identification of Resistant Animals

Location: Dale Bumpers Small Farms Research Center

2012 Annual Report


1a.Objectives (from AD-416):
The objective of this cooperative research project is to identify unique genetic markers related to internal parasitism of small ruminants.


1b.Approach (from AD-416):
Blood, tissue, and fecal samples will be collected from select populations of small ruminants with known parasitism history and(or) expected progeny differences (EPD) for internal parasitism and growth traits. Genomic DNA will be purified from buffy coat or tissue samples and screened for single nucleotide polymorphisms in candidate genes. Genes will include cytochrome P450 3A family, and prolactin. In addition, plasma will be screened for unique microRNA populations. Identification of unique polymorphisms will be related to fecal egg counts (FEC), FEC EPDs, growth traits, and reproductive success using regression models and analysis of variance.


3.Progress Report:

An experiment is being conducted to identify unique genetic markers related to internal parasitism of small ruminants. Blood, tissue, and fecal samples are currently being collected from select populations of small ruminants with known parasitism history and/or expected progeny differences (EPD) for internal parasitism and growth traits. Genomic DNA will be purified from buffy coat or tissue samples and screened for single nucleotide polymorphisms in candidate genes. Genes will include cytochrome P450 3A family, and prolactin. In addition, plasma will be screened for unique microRNA populations. Identification of unique polymorphisms will be related to fecal egg counts (FEC), FEC EPDs, growth traits, and reproductive success using regression models and analysis of variance. A graduate student has been selected and is currently collecting data and will begin sample analysis in fall 2012.


Last Modified: 11/22/2014
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