1a.Objectives (from AD-416):
Explore approaches that have the potential to be used to contribute to the efforts to eradicate Globodera nematode species from the Pacific Northwest.
1b.Approach (from AD-416):
We will develop methods and information that can be used for eradication of all three types of Globodera threatening the United State Potato Industry—G. pallida, G. rostochiensis and the new atypical Globodera.1. Identify/develop/evaluate superior trap crops that can stimulate a suicide hatch. 2. Explore ways to produce commercial amounts of hatching factors that could be delivered to infested fields through chemigation etc while growing some other crop. 3. Examine factors that influence hatching of the new Globodera and to determine to what extent it responds to the same trap crops and hatching factors as does G. pallida.
This project contributes to in-house project Objective 2: "Determine host resistance options, epidemiological parameters and develop diagnostic tests for emerging pests and pathogens of potato"; and Objective 3: "Elucidate genetic, molecular and biochemical factors governing host disease resistance and accumulation of select phytonutrients and vitamins." Globodera ellingtonae is a newly described species which is closely related to the regulated potato cyst nematodes (PCN). Because G. ellingtonae is not regulated, it provides a unique opportunity to test eradication strategies against this nematode in a field setting. In addition, since this species is newly described there is a need for information regarding the biology and pathogenicity of this nematode.
The effect of different overwintering conditions on diapause of G. ellingtonae eggs produced in the greenhouse was determined in 2013. Cysts containing eggs produced in the greenhouse were overwintered in either dry or wet conditions in the various environments. The cysts were recovered in the spring and hatch rates determined. In general, regardless of the environment in which greenhouse-produced eggs were placed, eggs did not hatch similarly to field-produced eggs. Among the overwintering treatments tested on greenhouse-produced eggs, overwintering in a wet environment in the field resulted in greater hatch rates. In an effort to identify hatching factors, a number of purified or partially purified compounds were evaluated for ability to stimulate hatch of G. ellingtonae. Over 150 dilutions of partially or fully compounds have been evaluated to date; this work is ongoing. Another ongoing project is the evaluation of litchi tomato as a trap crop for G. ellingtonae.