Developing the Genomic Infrastructure for Breeding Improved Black Raspberries (Cornell)
Horticultural Crops Research
2013 Annual Report
1a.Objectives (from AD-416):
Studying genotype by environment interaction on specific traits of interest in crosses involving diverse wild black raspberry germplasm. Gain a better understanding of consumer preferences for market expansion.
1b.Approach (from AD-416):
Studying genotype by environment interaction on specific traits of interest in crosses involving diverse wild germplasm. Mapping populations that segregate for a variety of traits have been generated using germplasm from the edges of the species native range. The populations will be planted and evaluated in New York, North Carolina and Oregon. Analyses of fruit chemistry will be made to examine total anthocyanins and phenolics. Evaluate transferability of SSR markers developed in black raspberry to red raspberry. SSR markers mined from black raspberry EST and genomic sequences will be evaluated for amplification and polymorphism in red raspberry by capillary electrophoresis. Polymorphism will be determined and added to linkage maps for those populations by UK collaborators.
This research was conducted in support of NP301 objective 1A of the parent project. Two black raspberry populations were established in the field at Cornell University’s New York State Agricultural Experiment Station (NYSAES) in Geneva, New York in 2012. In the spring of 2013, the plants were fertilized with 60 pounds equivalent of nitrogen using ammonium nitrate. The evaluations for traits among the plants in the populations began in early summer with ratings for many traits on each plant. The summer evaluations included floricane vigor, number of flowers and fruit on fruiting laterals and the length of those laterals at 3 locations on floricanes, the number of fruiting laterals on a representative floricane, the number of nodes below the top cluster of fruit that produced flowers, the date the first fruit was ripe, the fruit color, the fruit glossiness, the tightness of the fruiting clusters, the adherence or ease of picking of ripe fruit and the number of leaflets predominating on the leaves. Fruit began to ripen on June 29 and fruit sample were collected from each fruiting plant between then and July 12 for most individuals. A few were collected later as late fruit ripened in order to pick enough for analysis. This fruit was frozen for later analysis for sugars, acids, pigment content and other characters associated with fruit quality. A V-trellis for the field was also built and canes tied to the wires for the 2014 harvest season so that the plants can be more easily evaluated. Additional characteristics will be evaluated as the summer progresses including primocane vigor, number of primocanes, disease susceptibility. Additional plants from the populations that were not available for planting in 2012 were obtained from Oregon State and were planted in the field. These will be evaluated in future years. This evaluation on all the plants will be repeated in 2014.