2012 Annual Report 1a.Objectives (from AD-416): 1) Generate DNA sequence from 41 parents of a Nested Association Mapping population and selected RILs of NAM population 10,. 2)Generate DNA sequence from 25 ancestral soybean lines,. 3)Generate DNA sequence from 60 milestone cultivars representing 80 years of soybean improvement, and. 4)Generate RNASeq data on two NAM parents and selected RILs of the NAM population 10. 1b.Approach (from AD-416): Biological tissue will be collected by ARS and DNA/RNA will be provided to the collaborator. DNA resequencing will be conducted to a depth of 5 times coverage on all genotypes except select ancestral lines where deeper coverage may be required. RNASeq data will be generated on high and low yielding RIL lines of NAM population 10. DNA and RNA sequence information will be provided by the collaborator to ARS. 3.Progress Report: DNA was received from the ARS PI and libraries were prepared for sequencing. Preliminary sequence has been generated for three genotypes (IA3023, Richland and Mandarin) for analyses to assess DNA quality, sequence success, and depth of coverage. Analyses of this sequence indicated that the project was ready to proceed forward. DNA from forty genotypes has now been received from the ADODR and is in the process of being sequenced.