Project Number: 3655-21000-048-36
Project Type: Reimbursable

Start Date: Sep 01, 2011
End Date: Aug 31, 2015

Objective:
1) Develop a high-resolution cucumber inter-varietal linkage map placing at least 1,200 SSRs anchored with whole genome scaffolds. 2) Develop an efficient RNAi/transformation or VIGS. 3) Develop elite lines with DM and ZYMV resistance; identify molecular markers for the recessive DM and ZYMV resistance genes that can be used for MAS in breeding. 4) Identify candidate genes for downy-mildew and ZYMV resistance, and verify their functions.

Approach:
SSR markers were explored from cucumber whole genome sequence, and selected for mapping in an F2:3 mapping populations. Expect to place 1,200 SSRs on the map. 2. SNP discovery, SNP array development and use for fine mapping SNPs will be identified through alignment of whole genome sequences of two sequenced cucumber genotypes (9930 and Gy14), validated. SNP microarray will be manufactured through contract service to a company. The array will be used for SNP linkage mapping to identify markers linked with downy mildew and ZYMV disease resistance gene. 3. Cloning of ZYMV disease resistance locus from cucumber. Will use map-based cloning strategy.