1a.Objectives (from AD-416):
The objective of this project is to couple molecular genetics and physiology such that we can identify drought tolerant parental genotypes and those genotypes that have an increased likelihood of having unique drought tolerant alleles.
1b.Approach (from AD-416):
The approach of this research is to. 1)examine the extreme phenotypes in greater physiological detail under control and managed drought conditions,. 2)compare the responses of the extreme genotypes to other measures of drought tolerance,. 3)develop populations segregating for CID and NDFA (including developing RIL) based on genotypic and physiological criteria,. 4)phenotype segregating populations,. 5)initiate a mapping program to identify genes associated with CID and NDFA, and. 6)integrating global transcriptome analyses with physiological characterization and molecular marker development.
Based on stable isotope analyses from 2012 tissues, parental lines were selected for new crosses which are currently being made in the field. Genome wide association studies (GWAS) for carbon isotope discrimination were completed, and the GWAS analysis of ureide data is almost complete. F2 progeny from separate carbon isotope, nitrogen fixation, and ureide crosses were advanced in Puerto Rico, and F2:3 families for these traits were planted in Stoneville, MS, in April 2013. Ribonucleic acid was isolated, prepared for sequencing and delivered to the Midsouth Area Genomics Center for processing.