1a.Objectives (from AD-416):
Develop new sources of soybean resistance against root-knot and reniform nematode infection using biotechnology.
1b.Approach (from AD-416):
Identify genes in the nematode whose expression can be modulated in such a way as to inhibit root-knot and reniform nematode parasitism of soybean.
Extensive work was performed in characterizing the reniform nematode parasitism gene Rr-cle-1. The majority of work focused on identifying the tissues within the nematode that expressed the Rr-cle-1 gene. To perform this task, modifications were made to existing in situ hybridization protocols such that Rr-cle-1 expression was observed only in the dorsal esophageal gland cells of sedentary female reniform nematodes. Similar in situ hybridization experiments have been initiated for two new parasitism gene candidates. Transgenic soybean hairy roots were also produced, and are being propagated for nematode infection assays, that express hairpin-ribonucleic acid interference (RNAi) constructs that target the Rr-cle-1 gene