2012 Annual Report
1a.Objectives (from AD-416):
This application from the University of California Davis (UCD) seeks base funding to establish and operate the Mouse Metabolic Phenotyping Center (MMPC) at UCD. The MMPC-UCD will be a comprehensive resource for the phenotypic characterization of mouse models pertaining to the study of diabetes, obesity, and diabetic complications. Its mission will be to advance medical and biological research by providing the scientific community with standardized, high quality metabolic and physiologic phenotyping services for researchers utilizing the mouse for diabetes metabolic disease and diabetes-related research. The MMPC-UCD will specialize in performing detailed phenotypic evaluations of body composition, energy balance, metabolism, and diabetic complications in mice, notably cardiovascular (microvascular and macrovascular). In addition, the MMPC-UCD will offer structural pathology (non-invasive imaging technologies and conventional, experimental gross and microscopic histopathology), informatics, and biostatistics support necessary for analysis of diabetes, obesity, and related phenotypes. Finally, the MMPC-UCD will offer a web-driven, high-capacity, one-stop resource for customized and comprehensive phenotyping and analysis.
1b.Approach (from AD-416):
Briefly, the MMPC-UCD will be comprised of 5 Cores. Core A: Administrative; Core B: Animal Care; Core C: Metabolism and Endocrinology; Core D: Body Composition, Thermoregulation, and Food Intake Behavior; Core E: Complications and Pathology. Each of the Phenotyping Cores will be led by 2 UCD faculty members (a Core Director and Co-Director) and staffed by expert technicians and other staff in state-of-the-art technologies who will provide standardized assays to characterize physiologic, anatomic or pathological alterations that occur in mouse models of obesity and metabolism. The MMPC-UCD will provide detailed metabolic and physiologic tests and procedures performed with living animals and assays on tissues and specimens. The assays we offer will not only be broadly based but also include those beyond what would be possible or cost-effective in individual laboratories. Further, we will focus on technologies not yet widely available but are valuable for understanding metabolism and physiology.
This research contributes to objectives 3 and 5 of the in-house parent project. ARS scientists are collaborating with the Mouse Metabolic Phenotyping Center - University of California, Davis to set up the Body Composition, Thermoregulation, and Food Intake Behavior Core. Energy expenditure methods have been validated, and high-content results analysis algorithms instituted to provide reports on metabolic rate, activity level, and food intake in mouse metabolic models. In addition, animal body composition methods have been instituted and studies designed to test new methods for evaluation of temperature adaptation. The efforts are providing a strong infrastructure to test how diet, body fat, and genetics impact weight gain and obesity-associated (patho)physiology in animal models, information that may be translated to the human condition.