1a.Objectives (from AD-416):
1. To identify new sources for cold tolerance from exotic sorghum minicore germplasm through green house and field evaluation, and 2. To screen potential cold tolerance sources for Pythium spp. and analyze for tannin and other related quality traits.
1b.Approach (from AD-416):
Experimental Design: A sorghum minicore collection will be evaluated extensively for agronomic traits including resistance to biotic and abiotic stresses to identify accessions with desirable characteristics for use in crop improvement research and genomic studies. Seeds of all 245 germplasm (122-photo-sensitive; 123 photo-insensitive) were multiplied and now readily available for cold tolerance study. Flowering dates and yield will be recorded for all samples and seedlings will be harvested and analyzed for shoot and root weight. Grain quality traits, such as kernel physical traits (hardness, weight and diameter), total phenolic content and presence of tannins will be measured on samples.
Seeds of 96 lines selected from a sorghum “minicore germplasm collection” photoperiod sensitive and 118 photoperiod insensitive minicore were multiplied and were used in the cold tolerance screening for seedling traits. Germination was measured four times (7, 10, 13, 16 days after planting) in all 96 photoperiod sensitive minicore exotic germplasms and the emergence index was measured. Five samples showed 100% emergence within 7 days. Two of the samples were found to contain tannins. This research has identified potential new sources of cold tolerance germplasm which are currently being evaluated by breeders at Kansas State University.