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United States Department of Agriculture

Agricultural Research Service

Research Project: VALIDATION OF TESTING METHODS FOR THE DETECTION AND QUANTIFICATION OF ESCHERICHIA COLI 0157:H7,SALMONELLA SPP."TITLE CONTINUED IN AGREEMENT"

Location: Environmental Microbial and Food Safety Laboratory

Project Number: 1245-32420-005-35
Project Type: Reimbursable

Start Date: Jan 01, 2012
End Date: Jun 30, 2013

Objective:
The objective of this study is to evaluate TMECC and EPA pathogen detection methods in order to accurately determine the presence of populations of E. coli O157:H7, Salmonella, non-pathogenic E. coli, and fecal coliforms in ‘point of sale’ composts collected from commercial operations across the United States. Correlations between the presence of pathogens and several physico-chemical parameters (moisture, water activity, electrical conductivity, soluble carbon, pH, and carbon:nitrogen ratios) will be investigated.

Approach:
ARS will acquire the basic knowledge about the stresses that pathogenic E. coli or Salmonella strains undergo in compost and their prevalence in various types of compost. The carbon:nitrogen ratio will evaluated in commercial formulations. Based on these findings, compost formulations using specific C:N ratios will then be formulated to determine survival and inhibition of E. coli and Salmonella.

Last Modified: 4/20/2014
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