Location: Subtropical Plant Pathology Research
Project Number: 6618-22000-039-20
Start Date: Dec 06, 2011
End Date: Jan 31, 2014
1. Using the newly-developed super sensitive realtime polymerase chain reaction (PCR) to detect Las bacteria in the germplasms of citrus, and use the two effective chemicals coupled with thermal therapy to treat the budwoods and seeds to obtain real Las-free gerplasms. 2. Using realtime PCR and convential PCR to determine the type(s) of Las bacteria that can survive in Murraya plants and the dynamics of the bacteria in the plants, as well as their possibility to be transmitted to citrus by the asian citrus psyllids. 3. Optimizing the growth factors and conditions for improving the growth rate of Las bacterium in vitro, and knocking out and complementing some of the putative effector genes of Las. 4. Using the chemicals and thermotherapy device, we will apply and modify the application system for practical and cost effective control of citrus HLB.