1a.Objectives (from AD-416):
Identify and optimize formulation of phloem penetrants with natural antimicrobials/insect antifeedants.
1b.Approach (from AD-416):
Greenhouse and field trials of different formulations will be conducted on citrus plants. These formulations will include combinations of putative phloem penetrants and antimicrobial compounds. Uptake will be monitored throughout the plant using GC-MS protocols, and influence on citrus greening bacterium will be monitored through PCR detection of the citrus greening bacterium.
This project is related to Objective 3b: Develop novel control methods based on disrupting key processes in pest biology.
Combinations of antimicrobial products, penetrants and application techniques were tested in greenhouse and field experiments. Optimized methods for systemic delivery of antimicrobial products were developed and these conditions were shown to vary depending on the antimicrobial compound being used. Effect of treatments on the titer of the Citrus Greening associated bacterium are now being monitored in a year-long study in field citrus. In this work, foliar, soil and basal-bark applications strategies have been tested and effectiveness will be compared when experiments are complete.