Generation of Broad Spectrum, High Titer, Caprine Polyclonal Antisera Against Bovine Viral Diarrhea Virus (Bvdv)
2013 Annual Report
1a.Objectives (from AD-416):
The objective of this cooperative research project is to elicit the production of antisera in goats that recognizes a broad range of bovine viral diarrhea viruses (BVDV).
1b.Approach (from AD-416):
Antigenically and genetically diverse pestivirus strains will be selected. Inoculum will be prepared by growing selected viruses in tissue culture or by expressing proteins in a baculovirus vector. Inoculum will be delivered to goats in order to produce antibodies.
The group of viruses, known as bovine viral diarrhea viruses (BVDV), is made up of large number of viral strains that while similar in some aspects are very different in others. The difference, between strains, is known as heterogeneity. The level of heterogeneity among BVDV strains makes it difficult to develop diagnostic tests that are able to detect all BVDV strains (here after referred to as a broad spectrum BVDV test).
In order to produce reagents, to be used in diagnostic tests, that react to all BVDV strains, NADC researchers worked with a biologics company to expose goats to viral strains that encompass all the variations found among BVDV strains. The antisera produced by the goats in response to exposure to these viruses were tested and found to detect all the BVDV strains tested.