1a.Objectives (from AD-416):
The objective of this cooperative research project is to evaluate the potential of bioengineered yeast (Saccharomyces cerevisiae) expressing the horseradish peroxidase (HRP) enzyme for the remediation of odors from Concentrated Animal Feeding Operation (CAFO) lagoons.
1b.Approach (from AD-416):
Yeast (S. cerevisiae strains) expressing different isoforms of horse radish peroxidase will be evaluated for their ability to reduce p-cresol concentrations. Plasmids will be transformed into S. cerevisiae and assessed for HRP activity using the standard Luminol assay. Transformants expressing detectable HRP will then be tested for their ability to reduce p-cresol in CAFO lagoon water using GC/MS methods optimized for phenolic compounds by the Parker group at USDA-ARS. Yeast strains that are able to reduce p-cresol will be characterized for their viability and growth properties when they are expressing HRP.
ARS scientists at Clay Center, Nebraska and scientists with the University of Nebraska, Lincoln, Nebraska conducted a laboratory study to evaluate the efficacy of bioengineered yeast for expressing the horseradish peroxidase (HRP) enzyme. A method was developed for assessing the activity of the enzyme from several strains of yeast. The bioengineered yeast was effective in reducing certain odor concentrations in laboratory solutions.