1a.Objectives (from AD-416):
The objective of this cooperative research project is to first evaluate candidate compounds for their CYP-450 inhibition efficacy in important disease vectors like mosquitoes. The second is to identify novel compounds that synergize efficacy of pyrethroids in resistance population of mosquitoes/sand flies etc. The Cooperator has expertise IIBBL does not have in utilizing molecular tools and pyrethroid resistance colony of important vectors.
1b.Approach (from AD-416):
The general approach of this cooperative research project will allow ARS to acquire the basic knowledge of CYP-450 inhibitors against mosquitoes and bed bugs. This information will be used by both parties to jointly discover and develop chemicals for suppressing pyrethroid resistance in disease vectors. The Cooperator will provide molecular biology expertise and evaluate synergism of novel compounds against pyrethroid resistant insect populations.
The initial project plan was laid out in the first quarter of 2013. Based on the grant agreement, this physical year bioassay was developed by cooperator to evaluate pyrethroid resistance through CYP-450 isozymes from mosquitoes. Novel pyrethroids as well as CYP-450 inhibitors were synthesized and evaluated.