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United States Department of Agriculture

Agricultural Research Service

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Research Project: Targeting Root-Knot and Reniform Nematode Parasitism Genes to Develop Novel Resistance in Soybean

Location: Genetics and Precision Agriculture Research

Project Number: 6406-21000-014-35
Project Type: Reimbursable

Start Date: Oct 01, 2011
End Date: Sep 30, 2013

Objective:
Develop new sources of soybean resistance against root-knot and reniform infection using biotechnology.

Approach:
Esophageal gland cells of reniform nematode will be isolated from the vermiform and sedentary life-stages. The transcriptional profile of these gland cells will be determined via Next-Generation-Sequencing (NGS). Candidate parasitism genes will be identified from the NGS data by their homology with known parasitism genes from other plant-parasitic nematode species and by the presence/absence of specific protein motifs and domains. Candidate reniform nematode parasitism genes will be further characterized in an effort to determine whether they are potential targets for gene silencing using RNA-interference. If gland cells cannot be collected from each life-stage, then entire nematodes from the problematic life-stage will be sequenced and the common housekeeping genes filtered by comparison to transcripts found in the gland cells.

Last Modified: 10/1/2014
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