1a.Objectives (from AD-416):
Optimize and implement non-pheromonal blends for control and monitoring of the female navel orangeworm.
1b.Approach (from AD-416):
Using recently PMR-discovered efficacious volatile blends, personnel from the PMR laboratory will develop, analyze, and optimize blends for use in field trials. Personnel from Paramount Farming Company (cooperator and co-investigator) will assemble, distribute to almond and pistachio orchards, monitor, collect, and analyze traps with blend formulations.
ARS Completed: a) Using electroantennographic (EAG) analysis, 79 volatiles were identified from almond emissions, 48 volatiles were identified from pistachio emissions, and 37 volatiles were identified from associated fungal emissions. The EAGs were performed on antennae from both male and female navel orangeworm with sufficient replicates to provide publishable results and allow behavioral assays for blend candidacy; b) Over 20 new blends were formulated based on results from individual EAG results conducted on all blends. Candidate blends were subsequently used for field trapping and University of California, Riverside behavioral studies. Paramount Farming Company Completed: Due to extended temperature and moth pressures in orchards PFC personnel performed additional season-long trappings. Continued studies on lead blend and additional candidate blends/ formulations/ treatments were conducted. Suterra Completed: The blend (neat) was added into an initial membrane for testing. Recent work has included increasing the number of membranes for testing (total of six). This project directly relates to objective 3 of the parent project.