2012 Annual Report 1a.Objectives (from AD-416): Expedite production and laboratory efficacy testing of a novel anti-tick vaccine formulation as a tool for integrated approaches to keep the U.S. free of cattle fever ticks. 1b.Approach (from AD-416): In cooperation with the Texas Department of Agriculture and the Texas Animal Health Commission, funds from a sub-grant to the ARS will be used to accelerate a discovery research project for production and laboratory efficacy testing of Bm86-Texas anti-tick vaccine formulation as a tool for integrated approaches to keep the U.S. free of cattle fever ticks. 3.Progress Report: Sufficient antigen to conduct a 200-head cattle field study was produced, and a trial in white-tailed deer was initiated. This antigen was made available to the Texas Department of Agriculture and Texas Animal Health Commission for use as needed.