Location: Animal Parasitic Diseases
Project Number: 1245-31320-076-00
Start Date: Oct 11, 2011
End Date: Oct 10, 2016
Live Eimeria oocysts vaccines will be improved by testing the efficacy of and study immune responses elicited by gamma-irradiated Eimeria oocysts in protecting chickens against a coccidiosis. Optimum irradiation doses will be determined for the 3 major species of Eimeria that inhibits parasite development without affecting ability to elicit immunity. A novel delivery method by encapsulating Eimeria oocysts inside gelatin beads, and subsequent application onto feed for consumption by day-old chicks will be developed. Gel beads will be formulated to resist drying, and prepared containing low numbers of Eimeria oocysts. Day-old chicks will be fed gel beads, and tested at 4-5 weeks of age for immunity against coccidiosis. A rapid method for determining drug-sensitivity of Eimeria oocysts present in poultry facilities will be developed. Cell cultures containing various anti-coccidial compounds will be inoculated with field-strains of Eimeria sporozoites to determine sensitivity to these drugs.