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United States Department of Agriculture

Agricultural Research Service

Research Project: Development of Abiotic Stress Resistant Wheat Germplasm

Location: Hard Winter Wheat Genetics Research Unit

Project Number: 5430-21000-010-14
Project Type: Specific Cooperative Agreement

Start Date: Sep 15, 2011
End Date: Sep 14, 2016

Objective:
Develop wheat germplasm lines with resistance or tolerance to wheat rusts, Fusarium head blight, pre-harvest sprouting, heat stress, or drought stress. Construct and analyze populations to identify genes controlling these traits. Develop improved genotypic or phenotypic selection technologies for these traits.

Approach:
Association mapping populations have been constructed and are now being analyzed. For genotyping, we will use conventional SSRs, STS, and SNP markers and a genotyping-by-sequencing (GBS) approach suitable for the large complex genomes of wheat. Genotyping-by-sequencing uses restriction enzymes to produce a reduced representation of the genome that is then multiplex sequenced on the Illumina platform using DNA barcode adapters. We are able to identify tens of thousands of SNPs in each sample and use this information for genetic mapping and breeding. We will use the GBS data to identify SNP markers closely linked with the resistance genes while making background selection for the recurrent parent genotype. This marker assisted back-crossing approach will 1) identify molecular markers linked to traits of interest and 2) rapidly recover the elite wheat background for using these genes in breeding.

Last Modified: 9/2/2014
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