1a.Objectives (from AD-416):
Breed for high oleic acid, low linolenic acid and high oil soybean breeding lines/cultivars in maturity group II-IV.
1b.Approach (from AD-416):
The objective will be achieved crossing high oleic acid lines from Missouri with maturity group II-IV soybean cultivars and selecting high oleic acid progeny lines using marker assisted selection with melting curve assay on Lightcycler 480.
Five high oil, low linolenic acid and high-oleic acid soybean lines were crossed to high-yielding high-protein non-genetically modified organism (GMO) maturity group II-III soybean lines from Ohio in 2012. F1 and F2 seeds were produced from these crosses and the F2 seeds were planted in the field in 2013 to advance to F3 seeds. The crosses for high-oleic were made in Puerto Rico by a collaborator on the grant.
This project expands on the research on the improvement soybean quality aspects of the parent project (objective 4).